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sarcomeric alpha Actinin抗体 [EA-53]

Anti-sarcomeric alpha Actinin antibody [EA-53] used in IHC (Paraffin sections) (IHC-P). GTX29465
Anti-sarcomeric alpha Actinin antibody [EA-53] used in IHC (Paraffin sections) (IHC-P). GTX29465

目录号 GTX29465

文献引用 7
1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 EA-53

同种型

 IgG1

实验应用

 ICC/IF, IHC-P, IHC-Fr, IHC-Wm

种属反应

 Human, Mouse, Zebrafish
产品包装
500 μl (¥5300)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
ICC/IF Assay dependent
IHC-P 1:20‐1:30
IHC-Fr Assay dependent
IHC-Wm Assay dependent

Note :

IHC-P
Recommendations for antigen retrieval : EDTA buffer pH 8.0 at room temperature for 30 mins.
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

This antibody is specific for α-skeletal muscle actinin and α-cardiac actinin. It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non-sarcomeric muscle elements.

产品属性

形式

Liquid

存储溶液

Ascites

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

免疫原种属

Rabbit

免疫原

Purified rabbit skeletal α-actinin.

纯化方式

Unpurified

偶联

Unconjugated

RRID

AB_385929

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

actinin alpha 3

数据库

研究领域

產品图片

Anti-sarcomeric alpha Actinin antibody [EA-53] used in IHC (Paraffin sections) (IHC-P). GTX29465

GTX29465 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX29465 sarcomeric alpha Actinin antibody [EA-53].

Anti-sarcomeric alpha Actinin antibody [EA-53] used in IHC (Paraffin sections) (IHC-P). GTX29465

GTX29465 IHC-P Image

The data was published in the journal Cell Death Dis in 2018. PMID: 29371598

文献引用

Application Reference

Ma W et al. Nat Commun 2024; 15 (1) : 2137 N-Acetyltransferase 10 represses Uqcr11 and Uqcrb independently of ac4C modification to promote heart regeneration.
Gong R et al. Pharmacol Res 2021; : 105845 Loss of m6A methyltransferase METTL3 promotes heart regeneration and repair after myocardial injury.
实验应用 : IHC-Fr
种属反应 : Mouse
Bellmann J et al. Biomaterials 2019; 225 : 119537 A customizable microfluidic platform for medium-throughput modeling of neuromuscular circuits.
实验应用 : ICC/IF
种属反应 : Human
Williams AL et al. Physiol Genomics 2018; 50 (7) : 479-494 HIF1 mediates a switch in pyruvate kinase isoforms after myocardial infarction.
Milan M et al. Cell Death Dis 2018; 9 (2) : 108 Givinostat reduces adverse cardiac remodeling through regulating fibroblasts activation.
实验应用 : ICC/IF
种属反应 : Mouse
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产品评论

sarcomeric alpha Actinin antibody [EA-53] 目录号 GTX29465

Rating
( Average 5 based on 1 users reviews)
实验应用
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-sarcomeric alpha Actinin antibody [EA-53] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29465
Date : Anonymous submitted on 03-Aug-2018
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Mouse ES cell derived cardiomyocytes
Blocking : 5% BSA,1% goat serum, 0.2% gelatin, 0.1% Triton X-100, 0.1% NaN3, 25°C, 1Hr
Fixation : 4%PFA
Permeabilization /Antigen retrieval : 0.1% Triton X-100 / 5% BSA,1% goat serum, 0.2% gelatin, 0.1% Triton X-100, 0.1% NaN3
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Green: sarcomeric alpha actinin stained by sarcomeric alpha Actinin antibody [EA-53] (GTX29465) diluted at 1:1000. Blue: DAPI
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SDS
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产品包装 报价 (¥)
¥ 5300
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