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Summary Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein.

Summary DIRAS3 antibody is human specific.

Summary This antibody detects endogenous levels of BRCA1 only when phosphorylated at serine 1423.