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呼吸道合胞病毒研究

呼吸道合胞病毒研究

人类呼吸道合胞病毒 (Human Respiratory Syncytial Virus (RSV))是一种不容易检出的呼吸道病原体。经常导致婴儿、小孩、老人等免疫力较为低下或是需要长期照护的人群的死亡。RSV主要透过气溶胶或是近距离接触感染。一般人大多在出生后的两年内受到首次RSV感染,尔后在小孩与成人时期经常会发生再次感染。虽然与流行感冒病毒相比,RSV在临床上的症状没那么严重,但依然造成了全球每年约250,000名大人与60,000名儿童的死亡。

 

RSV属于肺炎病毒科(Pneumoviridae family),和同科病毒一样,外观型态多样(可能是长条型或球型)且外层包覆有套膜。其15.2kb的基因体搭载于负链单股RNA上,包含可翻译为11个蛋白质的10个基因(1),包括: 位于外层套膜上的用于附着的G蛋白(attachment glycoprotein,附着糖蛋白)、协助与宿主细胞膜进行融合的F蛋白(fusion glycoprotein,融合糖蛋白)以及SH蛋白(small hydrophobic protein,小疏水蛋白);位于内层套膜上的M蛋白(matrix protein, 基质蛋白);组成核壳体(ribonucleocapsid) 的N蛋白(nucleoprotein, 核蛋白)、P蛋白(phosphoprotein, 磷酸蛋白)与L蛋白(large protein, 大蛋白)、M2-1转录因子蛋白;另外还有M2-2调控蛋白以及非结构蛋白NS-1与NS-2。

 

RSV可分为A、B两个主要的抗原群体,两群体在蛋白质的表现上多有差异,其中主要用来定义抗原群体分类的是帮助附着宿主用的G蛋白。有趣的是,在激活中和抗体与制造有效疫苗对抗A和B两种RSV的试验中,反倒是融合前型态的F蛋白的theta位点被证明是最佳的RSV免疫抗原因子(2)。虽然价格高昂且半衰期短,但能对抗F蛋白的palivizuma是当下唯一获得核可上市的RSV治疗用单株抗体,主要施打对象为具有感染高风险性的婴幼儿。为了弥补当下治疗方法的不足,有许多直接对抗融合前型态F蛋白的单株抗体也加入了研发试验的行列中。

 

除了治疗方法之外,RSV检验方法也是临床科研的重点之一。尽管目前有数个用于检出RSV的方法,其中最值得信赖的还是相对耗时的鼻腔黏膜样本核酸测试(RT-PCR)。利用抗体测试进行实时检验,除了可以更快速的帮助诊断之外,未来也能协助进行更有效率的早期干预。因此,为了支持RSV基础科研,GeneTex开拓了一系列对抗G蛋白、F蛋白、N蛋白与M2蛋白的单克隆与多克隆抗体清单。另外,为了优化整体项目并增强未来RSV检出功效,GeneTex更进一步研发相关的重组兔单克隆抗体,如N蛋白抗体。这些N蛋白抗体不只具有优异的抗原亲和力,而且也通过了酶联免疫吸附测定(ELISA)、夹心法酶联免疫吸附测定(sandwich ELISA)以及侧向层析(lateral flow assay)的验证。此外,这些抗体还适用于Western blot、免疫沉淀(immunoprecipitation)与免疫组化(immunocytochemistry)等实验应用。

 

详情请参见下方重点产品与信息,也欢迎联系我们。


推荐使用于侧向流动分析之呼吸道合胞病毒抗体配对

 
Detection Antibody GTX636649
[HL1247]
GTX636650
[HL1248]
GTX636648
[HL1246]
GTX636650
[HL1248]
GTX636648
[HL1246]
GTX636649
[HL1247]
Capture Antibody GTX636648 [HL1246] GTX636649 [HL1247] GTX636650 [HL1248]
Sample Respiratory Syncytial Virus type A Nucleoprotein (GTX136751-pro)

 

GeneTex重组呼吸道合胞病毒抗体与他牌高引用克隆抗体之比较

Comparison of GeneTex’s Recombinant RSV Antibodies with other Market-leading Clones

 使用间接酶联吸附分析演示不同抗体对于A型 (图A) 与B型 (图B) 呼吸道合胞病毒核衣壳蛋白敏感度的比较: (1)蓝色系线条: GeneTex重组呼吸道合胞病毒核衣壳蛋白抗体GTX636648、 GTX636649以及GTX636650。 (2)红色系线条: 他牌呼吸道合胞病毒高引用克隆抗体。

 

推荐使用于夹心酶联吸附分析之呼吸道合胞病毒抗体配对

  Capture Antibody GTX636649 [HL1247]   Capture Antibody GTX636650 [HL1248]
  Detection Antibody GTX636648 [HL1246]   Detection Antibody GTX636648 [HL1246]

 

精选重组呼吸道合胞病毒抗体

Recombinant Comparable Abs 

  Cat. No. Product Name Clonality Applications
Recombinant GTX636647 Respiratory Syncytial virus Nucleoprotein antibody [HL1245] Rec Rb mAb WB, ELISA, Sandwich ELISA
Recombinant Comparable Abs GTX636648 Respiratory Syncytial Virus Nucleoprotein antibody [HL1246] Rec Rb mAb WB, ICC/IF, IP, ELISA, Lateral Flow,
Sandwich ELISA
Recombinant Comparable Abs GTX636649 Respiratory Syncytial Virus Nucleoprotein antibody [HL1247] Rec Rb mAb WB, IP, ELISA, Lateral Flow, Sandwich ELISA
Recombinant Comparable Abs GTX636650 Respiratory Syncytial Virus Nucleoprotein antibody [HL1248] Rec Rb mAb WB, ICC/IF, IP, ELISA, Lateral Flow, Sandwich ELISA
Recombinant GTX636705 Respiratory Syncytial Virus Nucleoprotein antibody [HL1291] Rec Rb mAb WB, ELISA, Sandwich ELISA
Recombinant Comparable Abs GTX636709 Respiratory Syncytial Virus Nucleoprotein antibody [HL1295] Rec Rb mAb WB, ICC/IF, ELISA, Sandwich ELISA
Recombinant Comparable Abs GTX636710 Respiratory Syncytial Virus Nucleoprotein antibody [HL1296] Rec Rb mAb WB, ICC/IF, ELISA, Sandwich ELISA
Recombinant Comparable Abs GTX636711 Respiratory Syncytial Virus type A Nucleoprotein antibody [HL1297] Rec Rb mAb WB, ICC/IF, IP, ELISA, Sandwich ELISA
Recombinant GTX637381 Respiratory Syncytial virus type B G protein antibody [HL1733] Rec Rb mAb WB, ICC/IF

 

重组呼吸道合胞病毒抗体之优异结合亲和力

Recombinant Comparable Abs 

  Cat. No. Clone EC50 RSV A NP
(GTX136751-pro)
EC50 RSV B NP
(GTX136931-pro)
Recombinant Comparable Abs GTX636648 HL1246 13.6 pM     15.4 pM
Recombinant Comparable Abs GTX636649 HL1247   3.8 pM     15.9 pM
Recombinant Comparable Abs GTX636650 HL1248   8.9 pM     10.1 pM
Recombinant GTX636705 HL1291 10.7 pM     19.0 pM
Recombinant Comparable Abs GTX636709 HL1295 19.8 pM   105.4 pM
Recombinant Comparable Abs GTX636710 HL1296 29.0 pM   170.8 pM
Recombinant Comparable Abs GTX636711* HL1297 16.7 pM 2854.8 pM

* GTX636711为A型呼吸道合胞病毒核衣壳蛋白之专一抗体

 

呼吸道合胞病毒重组核衣壳蛋白

Comparable Abs 

  Cat. No. Product Name Expression System Applications
Comparable Abs GTX136751-pro Respiratory Syncytial Virus type A Nucleoprotein, DDDDK tag HEK293 WB, ELISA, Lateral Flow, Sandwich ELISA
Comparable Abs GTX136931-pro Respiratory Syncytial Virus type B Nucleoprotein, DDDDK tag HEK293 WB, ELISA, Sandwich ELISA

 

 

重点产品
Recombinant Comparable Abs 

 

Respiratory Syncytial Virus type A Nucleoprotein, DDDDK tag (GTX136751-pro)

Respiratory Syncytial Virus type A Nucleoprotein, DDDDK tag (GTX136751-pro) Comparable Abs

Respiratory Syncytial Virus type B
Nucleoprotein, DDDDK tag (GTX136931-pro) Comparable Abs

 
Respiratory Syncytial Virus Nucleoprotein antibody [HL1248] (GTX636650)

Respiratory Syncytial Virus Nucleoprotein antibody [HL1248] (GTX636650)

RecombinantComparable Abs
Respiratory Syncytial Virus type A Nucleoprotein antibody [HL1297] (GTX636711)

Respiratory Syncytial Virus type A Nucleoprotein antibody [HL1297] (GTX636711)

RecombinantComparable Abs

 

参考文献:

(1) Nat Rev Microbiol. 2019 Apr;17(4):233-245.

(2) Pathogens. 2022 Nov 11;11(11):1324.

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