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Occludin antibody [MSVA-415M] HistoMAX™

HistoMAX
Anti-Occludin antibody [MSVA-415M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04461
Anti-Occludin antibody [MSVA-415M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04461
Anti-Occludin antibody [MSVA-415M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04461

Cat. No. GTX04461

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MSVA-415M

Isotype

 IgG1

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($679),
100 μl ($299)
See all Occludin products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes.

Negative Control

Colon: All non-epithelial and non-endothelial cells must not show any occludin staining.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ecombinant fragment of human Occludin protein (around aa 282-415)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

occludin , BLCPMG , PPP1R115 , PTORCH1

Cellular Localization

Cell membrane , Cell junction, tight junction

Background

This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]

Database

Research Area

DATA IMAGES

Anti-Occludin antibody [MSVA-415M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04461

GTX04461 IHC-P Image

IHC-P analysis of human clear cell ovarian carcinoma tissue section using GTX04461 Occludin antibody [MSVA-415M] HistoMAX™.
Clear cell ovarian carcinoma with moderate occludin staining of tumor cells.

Anti-Occludin antibody [MSVA-415M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04461

GTX04461 IHC-P Image

IHC-P analysis of human squamous epithelium tissue section using GTX04461 Occludin antibody [MSVA-415M] HistoMAX™.
In squamous epithelium occludin positivity is regularly seen but staining is reduced or absent in basal and superficial cell layers scaled.

Anti-Occludin antibody [MSVA-415M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04461

GTX04461 IHC-P Image

IHC-P analysis of human respiratory epithelium tissue section using GTX04461 Occludin antibody [MSVA-415M] HistoMAX™.
Membranous occludin immunostaining of respiratory epithelium scaled.

REFERENCE

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REVIEW

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Package List Price ($)
$ 679
$ 299