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SARS-CoV-2 (COVID-19) Spike FFPE 293T cell pellet block

SARS-CoV-2 (COVID-19) Spike FFPE 293T cell pellet block. GTX435640

Cat. No. GTX435640

Reference 2

Application

 IHC-P (cell pellet)

Species

 SARS Coronavirus 2
Package
1 block ($899)
See all SARS-CoV-2 (COVID-19) Spike products
PRODUCT

Summary

Block size is 2.2 x 2.2 x 0.5 cm (expected to yield ~40-50 sections of ~4-5 μm thickness), but can vary slightly. Cell pellet consists of 293T cells overexpressing SARS-CoV-2 spike protein (full-length, no tag) fixed with 4% PFA (15 min, room temp) 48 hrs post-transfection, and then embedded in paraffin. Please see the corresponding overexpression cell lysate  (GTX535664).

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

PROPERTIES

Storage

Cool dry at room temperature. 4ºC for long term storage.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike FFPE 293T cell pellet block. GTX435640

GTX435640 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) spike antibody detects SARS-CoV-2 (COVID-19) spike (full length) protein by immunohistochemical analysis.
Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Spike FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) spike (full length) protein stained by SARS-CoV-2 (COVID-19) spike antibody (GTX135356) diluted at 1:1000.
Red: SARS-CoV-2 (COVID-19) spike (full length) protein stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Sukhikh G et al. Viruses 2021; 13 (3) Vertical Transmission of SARS-CoV-2 in Second Trimester Associated with Severe Neonatal Pathology.
Sukhikh G et al. Viruses 2021; 13 (3) : 447 Vertical Transmission of SARS-CoV-2 in Second Trimester Associated with Severe Neonatal Pathology.
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REVIEW

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Package List Price ($)
$ 899