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Calbindin antibody [MSVA-471M] HistoMAX™

Orthogonal HistoMAX
Anti-Calbindin antibody [MSVA-471M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04454
Anti-Calbindin antibody [MSVA-471M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04454
Anti-Calbindin antibody [MSVA-471M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04454

Cat. No. GTX04454

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MSVA-471M

Isotype

 IgG2c

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($439),
100 μl ($299)
See all Calbindin products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:50-1:100

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

Kidney: A subset of the cells of distal tubuli should show an intense cytoplasmic calbindin 1 immunostaining.

Negative Control

Colon: Calbindin 1 immunostaining should be completely absent in all cells.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 7-96) of human CALB1 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calbindin 1 , CALB , D-28K

Cellular Localization

Nucleus , Cytoplasm, cytosol

Background

The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES

Anti-Calbindin antibody [MSVA-471M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04454

GTX04454 IHC-P Image

IHC-P analysis of human kidney cortex tissue section using GTX04454 Calbindin antibody [MSVA-471M] HistoMAX™.
A strong cytoplasmic Calbindin immunostaining occurs in a fraction of distal tubuli of the kidney.

Anti-Calbindin antibody [MSVA-471M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04454

GTX04454 IHC-P Image

IHC-P analysis of human colon mucosa tissue section using GTX04454 Calbindin antibody [MSVA-471M] HistoMAX™.
Calbindin immunostaining is absent in all cells of the colon mucosa.

Anti-Calbindin antibody [MSVA-471M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04454

GTX04454 IHC-P Image

IHC-P analysis of human cerebellum tissue section using GTX04454 Calbindin antibody [MSVA-471M] HistoMAX™.
Cerebellum molecular layer Purkinje cell layer granule cell layer white matter. A strong Calbindin staining of Purkinje cells and of associated axonal fibres is seen in the cerebrum.

REFERENCE

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REVIEW

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Package List Price ($)
$ 439
$ 299