产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Synthetic peptide of human EGFR VIII.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
EGFVviii , EGFR mutant , EGFR variant , EGFRv3
细胞定位
Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus,Secreted
功能与背景
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
数据库
研究领域
產品图片
![Anti-EGFR VIII antibody [HL2541] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638910](/templates/default/js/lightbox/images/loading.gif "Anti-EGFR VIII antibody [HL2541] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638910")
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GTX638910 ICC/IF Image
EGFR VIII antibody [HL2541] detects EGFR VIII protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: EGFR VIII stained by EGFR VIII antibody [HL2541] (GTX638910) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638910 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR VIII antibody [HL2541] (GTX638910) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638910 IHC-P (cell pellet) Image
EGFR VIII antibody [HL2541] detects EGFR VIII protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mock and transfected 293T cell pellet.Green: EGFR VIII stained by EGFR VIII antibody [HL2541] (GTX638910) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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![Anti-EGFR VIII antibody [HL2541] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638910](/upload/website/prouct_img/small/GTX638910/GTX638910_T-45124_20230825_ICC_IF_B_23090619_954.webp "Anti-EGFR VIII antibody [HL2541] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638910")
![Anti-EGFR VIII antibody [HL2541] used in Western Blot (WB). GTX638910](/upload/website/prouct_img/small/GTX638910/GTX638910_45187_20231013_WB_multiple_B_23101921_130.webp "Anti-EGFR VIII antibody [HL2541] used in Western Blot (WB). GTX638910")
![Anti-EGFR VIII antibody [HL2541] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX638910](/upload/website/prouct_img/small/GTX638910/GTX638910_T-45124_20231020_IHC-P_cell_pellet_B_23102401_176.webp "Anti-EGFR VIII antibody [HL2541] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX638910")
![Anti-EGFR VIII antibody [HL2541] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638910](/upload/website/prouct_img/normal/GTX638910/GTX638910_T-45124_20230825_ICC_IF_B_23090619_954.webp "Anti-EGFR VIII antibody [HL2541] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638910")
![Anti-EGFR VIII antibody [HL2541] used in Western Blot (WB). GTX638910](/upload/website/prouct_img/normal/GTX638910/GTX638910_45187_20231013_WB_multiple_B_23101921_130.webp "Anti-EGFR VIII antibody [HL2541] used in Western Blot (WB). GTX638910")
![Anti-EGFR VIII antibody [HL2541] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX638910](/upload/website/prouct_img/normal/GTX638910/GTX638910_T-45124_20231020_IHC-P_cell_pellet_B_23102401_176.webp "Anti-EGFR VIII antibody [HL2541] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX638910")
