产品说明
摘要
GeneTex's ELISA Blocking Buffer is a protein buffer that effectively blocks plate binding sites that remain in polystyrene microplate wells following initial coating steps in ELISA procedures. The ready-to-use buffer is optimized and ready to use for sandwich ELISA procedures.
Instructions for use in an ELISA : Following the incubation with a coating antibody, aspirate the coating antibody from the plate and add 300 μL of ELISA Blocking Buffer to each well. Cover the plate with a plate sealer and incubate for a minimum of 1 hour at room temperature (22-25ºC). Aspirate blocking buffer and proceed with the ELISA procedure or dry the plate for future use.
Plate Drying : Plates should be dried uncovered, at room temperature, for a minimum of 1 hour. For optimal drying, plates should not be stacked and humidity should be at 30-40%. If plates are stacked, or humidity is high, the drying time should be increased. Once drying is complete, seal each plate in a foil pouch with a desiccant. When sealed with a desiccant, plates may be stored at 2-8ºC for up to 6 months.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
产品属性
形式
Liquid
存放说明
Keep as concentrated solution, aliquot and store at 4ºC. DO NOT FREEZE.
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
ELISA blocking buffer , ELISA blocking solution , ELISA plate blocker
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