产品说明
摘要
Proteinase K is specifically formulated to achieve proteolytic digestion of formalin fixed, paraffin embedded tissue sections prior to performing in situ hybridization procedures. Proteolytic digestion of formalin fixed tissues improves the accessibility of target DNA to biotinylated probes, which results in better in situ staining.
产品应用
应用说明
Preparation of working solutions :
1. Place 100μl Proteinase K solution in a glass tube, and 2.4ml distilled water and mix.
Protocol Recommendations :
1. Optimal digestion conditions will vary depending on fixation conditions and should be optimized by the end user.
2. Incubation of tissue sections for 10 - 30 minutes at 37ºC is sufficient for in situ.
产品属性
形式
Liquid
存放说明
Store at 2-8ºC. Proteinase K is stable for 6 months. For long term storage, aliqout Proteinase K into smaller sizes and store at -20ºC. Do not use after expiration date printed on label.
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
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