Oxytocin ELISA kit

This immunoassay kit allows for the in vitro quantitative determination of general Oxytocin concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oxytocin. During the reaction, Oxytocinin the sample or standard competes with a fixed amount of biotin-labeled Oxytocin for sites on a pre-coated Monoclonal antibody specific to Oxytocin. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Oxytocin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Detect Range : 0.156-10 ng/m
Sample Type : Serum, plasma, tissue homogenates and other biological fluids.
Sample Volume : 100ul
Assay Time : 3-5h
Detection wavelength : 450 nm

Content Package Storage Assay plate 1 plate -20 ºC Standards 2 vials -20 ºC Sample Diluent 20ml Please see the label of the vial Assay Diluent A 10ml Please see the label of the vial Assay Diluent B 10ml Please see the label of the vial Detection Reagent A 60μl -20 ºC Detection Reagent B 120μl -20 ºC 25 x concentrated Wash Buffer 30ml Please see the label of the vial Substrate 10ml 4 ºC Stop Solution 10ml Please see the label of the vial Plate sealer 5 strips Room temperature