Calnexin抗体 [N3C2], Internal

Name: Calnexin抗体 [N3C2], Internal
Brand: GeneTex
SKU: GTX101676
Price: 4000.00 RMB

Anti-Calnexin antibody [N3C2], Internal used in Western Blot (WB). GTX101676

Anti-Calnexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101676

Anti-Calnexin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101676

目录号 GTX101676

文献引用 8

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P
种属反应	Human, Mouse, Rat
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 Calnexin 的产品

View product citations for antibody GTX101676 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

68 kDa. ( Note )

阳性对照

NIH-3T3 , PC-12 , Rat2 , 293T

预测种属反应

Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Calnexin. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1949820

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

calnexin , CNX , IP90 , P90

细胞定位

Endoplasmic reticulum membrane , Melanosome

功能与背景

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

数据库

Gene ID: 821 CANX
UniProt: P27824 CANX

研究领域

產品图片

GTX101676 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101676 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX101676 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX101676 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101676 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101676 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101676 IHC-P Image

GTX101676 ICC/IF Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

GTX101676 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101676 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody (GTX101676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101676 WB Image

The data was published in the journal Nat Commun in 2020.PMID: 32111843

GTX101676 WB Image

The data was published in the journal Cells in 2019. PMID: 31775322

文献引用

Application Reference

Nazma F. Ilahibaks et al. J of Extracellular Bio 2023; 2 : e97 Extracellular vesicle-mediated protein delivery to the liver

实验应用 : WB

van de Wakker SI et al. Eur J Pharm Biopharm 2021; Influence of short term storage conditions, concentration methodsand excipients on extracellular vesicle recovery and function.

实验应用 : WB

种属反应 : Human

Wijenayake S et al. PLoS One 2021; 16 (9) : e0257633 Comparison of methods for pre-processing, exosome isolation, and RNA extraction in unpasteurized bovine and human milk.

实验应用 : WB

种属反应 : Human

de Jong OG et al. Nat Commun 2020; 11 (1) : 1113 A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA.

实验应用 : WB

种属反应 : Human

van den Hoogen P et al. PLoS One 2019; 14 (12) : e0227283 Potential of mesenchymal- and cardiac progenitor cells for therapeutic targeting of B-cells and antibody responses in end-stage heart failure.

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Application Reference

Nazma F. Ilahibaks et al. J of Extracellular Bio 2023; 2:e97 Extracellular vesicle-mediated protein delivery to the liver

实验应用 : WB

van de Wakker SI et al. Eur J Pharm Biopharm 2021; Influence of short term storage conditions, concentration methodsand excipients on extracellular vesicle recovery and function.

实验应用 : WB

种属反应 : Human

Wijenayake S et al. PLoS One 2021; 16 (9):e0257633 Comparison of methods for pre-processing, exosome isolation, and RNA extraction in unpasteurized bovine and human milk.

实验应用 : WB

种属反应 : Human

de Jong OG et al. Nat Commun 2020; 11 (1):1113 A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA.

实验应用 : WB

种属反应 : Human

van den Hoogen P et al. PLoS One 2019; 14 (12):e0227283 Potential of mesenchymal- and cardiac progenitor cells for therapeutic targeting of B-cells and antibody responses in end-stage heart failure.

Ilahibaks NF et al. Cells 2019; 8 (12) Biofabrication of Cell-Derived Nanovesicles: A Potential Alternative to Extracellular Vesicles for Regenerative Medicine.

Verdera HC et al. J Control Release 2017; (Epub) Cellular uptake of extracellular vesicles is mediated by clathrin-independent endocytosis and macropinocytosis.

实验应用 : WB

种属反应 : Human

Sever L et al. Dev Comp Immunol 2014; 42 (2):211-9 Rainbow trout (Oncorhynchus mykiss) contain two calnexin genes which encode distinct proteins.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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	http://www.neobioscience.com
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Calnexin抗体 [N3C2], Internal

目录号 GTX101676

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX101676 WB Image

GTX101676 WB Image

GTX101676 WB Image

GTX101676 IHC-P Image

GTX101676 IHC-P Image

GTX101676 IHC-P Image

GTX101676 WB Image

GTX101676 IHC-P Image

GTX101676 IHC-P Image

GTX101676 WB Image

GTX101676 IHC-P Image

GTX101676 ICC/IF Image

GTX101676 WB Image

GTX101676 IHC-P Image

GTX101676 WB Image

GTX101676 WB Image

GTX101676 WB Image

文献引用

Application Reference

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