产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HCT116 (30uM cisplatin treatment for 24hr) , PC-12 , Rat2 , 293T , A431 , HeLa , HepG2
产品说明
This antibody is specific for human PARP1 protein, and it does not cross react with human PARP2 and PARP3 protein.
预测种属反应
Bovine, Chicken(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.3 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1241155
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1
细胞定位
Nucleus,Nucleolus
功能与背景
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX100573 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100573 WB Image
PARP1 antibody detects PARP1 protein by western blot analysis. A. 30 μg HCT116 whole cell lysate/extract (untreated) B. 30 μg HCT116 whole cell lysate/extract (30 μM cisplatin treatment for 24hr) 7.5% SDS-PAGE PARP1 antibody (GTX100573) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100573 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with PARP antibody (GTX100573) diluted at 1:2000 and competitor's antibody (#9542) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX100573 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:2000.
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GTX100573 ChIP assay Image
Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.
ChIP experiment and primer designs are based on BMC Mol Biol. 2006 Dec 22;7:48.
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GTX100573 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100573 IHC-P Image
PARP antibody detects PARP protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. PARP antibody (GTX100573) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100573 ICC/IF Image
PARP antibody detects PARP protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PARP protein stained by PARP antibody (GTX100573) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 μm.
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GTX100573 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100573 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100573 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100573 WB Image
The data was published in the journal Sci Rep in 2017.PMID: 28928421
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GTX100573 WB Image
The data was published in the journal Nat Commun in 2016.PMID: 27703167
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GTX100573 WB Image
The data was published in the journal Oncotarget in 2016. PMID: 26716417
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GTX100573 WB Image
The data was published in the journal PLoS Genet in 2016. PMID: 27626929
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GTX100573 WB Image
The data was published in the journal PLoS Genet in 2016. PMID: 27626929
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GTX100573 WB Image
The data was published in the journal Evid Based Complement Alternat Med in 2012. PMID: 23091557
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GTX100573 WB Image
The data was published in the journal PLoS Genet in 2016. PMID: 27626929
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GTX100573 WB Image
The data was published in the journal Oncotarget in 2015. PMID: 26160843
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GTX100573 WB Image
The data was published in the 2022 in Cancers (Basel). PMID: 35008409
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GTX100573 WB Image
The data was published in the 2022 in FEBS Open Bio. PMID: 34856073
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GTX100573 IP Image
The data was published in the journal Nat Commun in 2016.PMID: 27703167
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GTX100573 ChIP assay Image
The data was published in the journal PLoS Genet in 2016. PMID: 27626929
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文献引用
产品评论
PARP antibody
目录号 GTX100573
Rating
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( Average 5 based on 2 users reviews)
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实验应用
Western Blot(WB)
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( Average 5 based on 2 users reviews)
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Date :
Anonymous submitted on 31-Jan-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
iPSC
Amount used :
25 μg
Blocking :
5% milk, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 26-May-2018
Application Tested :
WB
Sample Species :
Hu
Sample :
MG63 cells
Amount used :
30 μg
Primary Antibody :
1:1000, 4°C, 16Hr
条带说明 :
Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysate