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甲型流感和乙型流感重组单克隆抗体

GeneTex 推出一系列针对甲型流感病毒 (Flu A) 和乙型流感病毒 (Flu B) 核蛋白 (NP) 的重组兔单克隆抗体,以扩展其已经广泛的流感病毒研究试剂目录。 这些高质量的抗体通过验证可用于侧向流动分析(LFA;图 1),推荐用于 LFA 的抗体对列于表 1 与表 2 中。

Influenza A and B Recombinant Monoclonal Antibodies
Influenza A and B Recombinant Monoclonal Antibodies


图 1. 在侧流分析中检测 Flu A 和 Flu B NP。
使用指定的重组兔单克隆抗体对,通过横向流动测定法检测源自甲型流感病毒 (A) 和乙型流感病毒 (B) 的重组 NP。 HL1078:甲型流感病毒核蛋白抗体 [HL1078] (GTX636199); HL1089:甲型流感病毒核蛋白抗体 [HL1089] (GTX636247); HL1103:甲型流感病毒核蛋白抗体 [HL1103] (GTX636318); HL1068:乙型流感病毒核蛋白抗体 [HL1068] (GTX636099); HL1069:乙型流感病毒核蛋白抗体 [HL1069] (GTX636100); HL1073:乙型流感病毒核蛋白抗体 [HL1073] (GTX636194); 样品:甲型流感病毒核蛋白(NP)(A/WSN/1933(H1N1)),DDDDK 标签(GTX135868-pro); 样品:乙型流感病毒核蛋白 (NP) (B/Sydney/3/2004),DDDDK 标签 (GTX135867-pro)。(C) 使用指定的重组兔单克隆抗体对,通过侧向流动测定法检测来自甲型流感病毒 (左) 或乙型流感病毒 (右) 的重组核蛋白 (NP)。

表 1. 用于侧流分析的 Flu A 抗体对

Detection Capture  
Clone HL1103 (GTX636318) Clone HL1089 (GTX636247)  

 

表 2. 用于侧流分析的 Flu B 抗体对

Detection Capture  
Clone HL1068 (GTX636099) Clone HL1069 (GTX636100)  

 

 

此外,这些抗体可作为夹心 ELISA (sELISA) 的配对使用,与其他市场领先的克隆相比,具有低皮摩尔范围的灵敏度和优异的 EC50 值(图 2)。


图 2. 在 ELISA 分析中检测甲型流感病毒和乙型流感病毒 NP。
通过 sELISA 分析源自不同甲型流感病毒和乙型流感病毒株的重组 NP,使用 (A) Flu A NP 抗体对 GTX636318GTX636247 或(B) Flu B NP 抗体对 GTX636099GTX636100;间接 ELISA 证明 (C) Flu A NP 抗体 GTX636199GTX636247GTX636318 以及 (D) Flu B NP 抗体 GTX636100GTX636099GTX636194 克隆的 EC50 值优于竞争对手的克隆。

 

重要的是,至少有 16 种 Flu A 毒株和 9 种 Flu B 毒株可以通过 ELISA、蛋白质印迹和免疫细胞化学检测到,包括 Flu A H1N1 和 H3N2 亚型(图 3),与竞争对手的克隆相比,这些重组抗体显示出更高的敏感性(图 4),该数据证明了这些重组抗体在诊断应用中的潜在价值。


图 3. 流感病毒株分析。
利用夹心 ELISA 检测感染不同病毒株后细胞裂解物中的 NP,使用Flu A NP 抗体对 GTX636318GTX636247 检测(A) 甲型流感病毒亚型 H1N1 与 (B) 亚型 H3N2 或(C)使用 Flu B NP 抗体对 GTX636099GTX636100。 (D) Flu A NP 抗体 GTX636199 通过蛋白质印迹识别感染细胞裂解物中各种 Flu A 亚型的 NP。使用 Flu A NP 抗体 GTX636247 (E) 或 Flu B NP 抗体 GTX636194 (F) 对 NP 基因转染的 293T 细胞进行免疫细胞化学染色。

 

 


图 4. GeneTex 重组抗体与市场领先克隆的比较。
与竞争对手的克隆相比,间接 ELISA 检测显示 (A) Flu A NP 抗体 GTX636318GTX636247GTX636199 以及 (B) Flu B NP 抗体 GTX636099GTX636100GTX636194 的敏感性较好。

 

下面显示了每种特色重组蛋白和抗体的完整应用列表(表 3-4)。

表 3. 用于 Flu A 研究的精选重组蛋白和抗体

  Product Name Clonality Applications Cat. No.
  Influenza A virus Nucleoprotein (A/Kansas/2017/H3N2), DDDDK Tag   ELISA, Lateral Flow GTX135903-pro
  Influenza A virus Nucleoprotein (A/California/2009 (H1N1)), DDDDK Tag   ELISA GTX135904-pro
  Influenza A virus Nucleoprotein (A/Hong Kong/2671/2019 (H3N2)), DDDDK Tag   ELISA, Lateral Flow GTX136317-pro
Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag   ELISA, Lateral Flow, Sandwich ELISA GTX135868-pro
Influenza A virus Nucleoprotein antibody [GT1236] Ms mAb WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Sandwich ELISA GTX629633
Influenza A virus Nucleoprotein antibody [GT778] Ms mAb WB GTX629544
  Influenza A virus Nucleoprotein antibody [HL1078] Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA GTX636199
    Influenza A virus Nucleoprotein antibody [HL1089] Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA, IHC-P (cell pellet) GTX636247
Influenza A virus Nucleoprotein antibody [HL1098] Rb mAb WB, ICC/IF, ELISA, Sandwich ELISA GTX636282
  Influenza A virus Nucleoprotein antibody [HL1103] Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA, IHC-P (cell pellet) GTX636318
Influenza A virus Nucleoprotein antibody [HL1321] Rb mAb WB GTX636739
Influenza A virus Nucleoprotein antibody [HL1323] Rb mAb WB GTX636741
  Influenza A virus Nucleoprotein antibody Rb pAb WB, ICC/IF, IHC-P, IP, ELISA, Sandwich ELISA GTX125989
Influenza A virus H1N1 HA (Hemagglutinin) antibody Rb pAb WB, ICC/IF, IHC-P, ELISA GTX127357
  Influenza A virus H1N1 HA (Hemagglutinin) antibody Rb pAb WB GTX127295
  Influenza A virus H1N1 HA (Hemagglutinin) antibody [GT223] Ms mAb WB, ICC/IF GTX629750
Influenza A virus H1N1 HA (Hemagglutinin) antibody [GT521] Ms mAb WB GTX629746
Influenza A virus H1N1 NA (Neuraminidase) antibody Rb pAb WB, ICC/IF GTX125974
  Influenza A virus H1N1 NA (Neuraminidase) antibody [GT172] Ms mAb WB GTX629695
Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] Ms mAb WB, ICC/IF GTX629696
Influenza A virus H1N1 NA (Neuraminidase) antibody [HL1108] Rb mAb WB, ICC/IF GTX636323
Influenza A virus H3N2 HA (Hemagglutinin) antibody Rb pAb WB, IHC-P GTX127363
Influenza A virus H10N3 HA (Hemagglutinin) antibody [HL1559] Rb mAb ICC/IF, IHC-P (cell pellet) GTX637034
  Influenza A virus M1 (Matrix protein) antibody Rb pAb WB, ICC/IF, IHC-Fr GTX125928
Influenza A virus M1 (Matrix protein) antibody Rb pAb WB, ELISA GTX127356
Influenza A virus M1 (matrix protein) antibody [HL1273] Rb mAb WB, ICC/IF, IHC-P (cell pellet) GTX636675
Influenza A virus M1 (matrix protein) antibody [HL1275] Rb mAb WB, ICC/IF, IHC-P (cell pellet) GTX636677
Influenza A virus M2 (Matrix protein) antibody Rb pAb WB, ICC/IF, IHC-Fr GTX125951
  Influenza A virus NS1 (nonstructural protein) antibody Rb pAb WB, ICC/IF, IHC-P GTX125990
Influenza A virus NS1 (nonstructural protein) antibody [GT1653] Ms mAb WB GTX633685
  Influenza A virus NS1 (nonstructural protein) antibody [GT1943] Ms mAb WB GTX633686
  Influenza A virus NS2 / NEP antibody Rb pAb WB, ICC/IF GTX125953
Influenza A virus NS2 / NEP antibody Rb pAb WB GTX125952
Influenza A virus PA protein antibody Rb pAb WB, ICC/IF GTX118991
Influenza A virus PA protein antibody Rb pAb WB, ICC/IF GTX125932
Influenza A virus PA protein antibody Rb pAb WB, ICC/IF GTX125933
Influenza A virus PA protein antibody [HL1388] Rb mAb WB GTX636828
Influenza A virus PB1 protein antibody Rb pAb WB, ICC/IF GTX125923
Influenza A virus PB1 protein antibody [HL1714] Rb mAb WB GTX637313
Influenza A virus PB1 protein antibody [HL1715] Rb mAb WB GTX637314
  Influenza A virus PB2 protein antibody Rb pAb WB, ICC/IF GTX125926
Influenza A virus PB2 protein antibody Rb pAb WB, ICC/IF GTX125925

 

表 4. 用于 Flu B 研究的精选重组蛋白和抗体

  Product Name Clonality Applications Cat. No.
  Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag   ELISA, Lateral Flow GTX135867-pro
  Influenza B virus Nucleoprotein (B/Victoria/02/1987), DDDDK Tag   ELISA, Lateral Flow GTX136277-pro
  Influenza B virus Nucleoprotein antibody [HL1068] Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA GTX636099
  Influenza B virus Nucleoprotein antibody [HL1069] Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA GTX636100
  Influenza B virus Nucleoprotein antibody [HL1073] Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA GTX636194
  Influenza B virus Nucleoprotein antibody [GT371] Ms mAb WB, ICC/IF, ELISA, Sandwich ELISA GTX629882
  Influenza B virus Nucleoprotein antibody [GT382] Ms mAb WB, ICC/IF, ELISA, Sandwich ELISA GTX629883
Influenza B virus Nucleoprotein antibody Rb pAb WB, ICC/IF, ELISA, Sandwich ELISA GTX128538
Influenza B virus Nucleoprotein antibody Rb pAb WB, ICC/IF, ELISA, Sandwich ELISA GTX128539
  Influenza B virus Nucleoprotein antibody Rb pAb WB, IHC-P (cell pellet) GTX135819
  Influenza B virus Hemagglutinin (HA) antibody Rb pAb WB, ICC/IF GTX128543
  Influenza B virus Hemagglutinin (HA) antibody Rb pAb WB, ICC/IF GTX128542
  Influenza B virus M (Matrix protein) antibody Rb pAb WB, ICC/IF GTX128537
  Influenza B virus M (Matrix protein) antibody Rb pAb WB GTX128536
  Influenza B virus Neuraminidase (NA) antibody Rb pAb ICC/IF GTX128541
  Influenza B virus Neuraminidase (NA) antibody Rb pAb ICC/IF GTX128540
Influenza B virus Neuraminidase (NA) antibody [HL1476] Rb mAb WB, ICC/IF GTX636950
  Influenza B virus antibody Rb pAb WB GTX128544

 

表 5. 用于流感研究的其他重组蛋白和抗体

  Product Name Clonality Applications Cat. No.
  Influenza B virus Nucleoprotein antibody [GT382] Ms mAb WB, ICC/IF, ELISA, Sandwich ELISA GTX629883
  Avian Influenza A virus H5N1 HA (Hemagglutinin) antibody [1D10] Ms mAb WB, ICC/IF GTX632358
  Avian Influenza A virus H5N1 NA (Neuraminidase) antibody Rb pAb WB GTX127984
  Avian Influenza A virus H5N3 HA (Hemagglutinin) antibody Rb pAb WB GTX127299
Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] Rb mAb WB, IHC-P (cell pellet) GTX637025
Avian Influenza A virus H7N7 HA (Hemagglutinin) antibody Rb pAb WB GTX127303
  Avian Influenza A virus H7N7 HA (Hemagglutinin) antibody Rb pAb WB GTX127302
  Avian Influenza A virus H9N2 HA (Hemagglutinin) antibody Rb pAb WB GTX127307
  Avian Influenza A virus H9N2 HA (Hemagglutinin) antibody Rb pAb WB GTX127306
  Avian Influenza A virus H9N2 HA (Hemagglutinin) antibody Rb pAb WB GTX127305
  Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody Rb pAb WB GTX135762
Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1342] Rb mAb WB, ICC/IF, IHC-P (cell pellet) GTX636762
Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] Rb mAb WB, ICC/IF, IHC-P (cell pellet) GTX636763